Abstract

Developed very impressionable, discriminating, and exact extreme-efficiency liquid chromatographytandem bulk spectrometry form for measurement of Emtricitabine and Tenofovir Alafenamide in human plasma. Emtricitabine15ND2 Tenofovir D5 was used as a within the standard (IS). The samples were divided on Phenomenex Kinetex C18 5 μm 100A, 100*4.6 mm procession utilizing Acetonitrile: 0.2% formic acid, 60:40 V/V as movable state, the retention period of Emtricitabine: 1.36 minutes (±0.5 minutes), Emtricitabine 15ND2: 1.36 minutes (±0.5 minutes), Tenofovir Alafenamide: 1.41 minutes (±0.5 minutes) and Tenofovir Alafenamide D5: 1.41 minutes (±0.5 minutes) individually. The precursor to product ion evolutions of Emtricitabine 130.200/248.200 m/z and Tenofovir 176.200/477.250 m/z were used to discover and compute Emtricitabine and Tenofovir Alafenamide and Emtricitabine 15ND2 Tenofovir D5 individually. A Shimadzu LC-MS-8045 wholeequipped accompanying vehicle propelled by ejection of pressurized gas or liquid ion spray (TIS) source and conducted in multiple reactions monitoring (MRM) style was pre-owned for the study.

Keyworde: Emtricitabine, LC-MS/MS, Chromatography, Extraction, Tenofovir, Antipsychotic, Depot Drug, HIV

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